Composite

Part:BBa_K086003:Design

Designed by: Gairik Sachdeva   Group: iGEM08_IIT_Madras   (2008-10-28)


modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cleaned of RE sites


Source

LacO conserved sequence and σ24 binding conserved sequence

References